Characterization of Armillaria heimii from Africa

Characterization of Armillaria isolates from tea (Camellia sinensis) in Kenya.

Armillaria is a primary root rot pathogen of tea (Camellia sinensis) in Kenya. The main species presently described in this country are A. mellea and A. heimii. A survey covering fourteen districts of Kenya was carried out and forty-seven isolates of Armillaria collected. Cultural morphology, rhizomorph characteristics, somatic incompatibility and features of basidiomata were used to characteri...

Purification, characterization and cDNA cloning of an endo-exonuclease from the basidiomycete fungus Armillaria mellea.

We have purified an endo-exonuclease from the fruiting body of the basidiomycete fungus Armillaria mellea by using an ethanol fractionation step, followed by two rounds of column chromatography. The enzyme had an apparent molecular mass of 17500 Da and was shown to exist as a monomer by gel-filtration analysis. The nuclease was active on both double-stranded and single-stranded DNA but not on R...

Purification and characterization of a ribonuclease from the wild edible mushroom Armillaria luteo-virens.

A 15 kDa ribonuclease (RNase) was purified from dried fruiting bodies of the wild edible mushroom Armillaria luteo-virens. The simple 4-step purification protocol involved ion-exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion-exchange chromatography on SP-Sepharose and a final gel filtration by FPLC on Superdex-75. The RNase was unadsorbed on Affi-gel ...

ISOLATION AND CHARACTERIZATION OF SIX HIGHLY INTRINSIC FLUORESCENT METABOLITES IN ARMILLARIA MELLEA (VAHL. EX. FR.) KARST

Six fluorescent compounds were isolated and characterized in rhizomorph of A. mellea and were presented first as: F , F , F , MF , MF and MF . These compounds differed by Rf on chromatoplaque, max in UV spectra and ex, em and max in fluorescence spectra. Their GC-MS analysis indicated that F has a phenolic moiety and the other five are the benzenic derivatives. F compound was more f...

Short title: Kim et al: Characterization of North American Armillaria species Characterization of North American Armillaria species by DNA content and RFLP analysis